Atletico Madrid lost 2-1 to Levante, forcing the out of La Liga title race.
Both Barcelona and Real Madrid won their matches.
Barcelona is atop the table with 88 points, followed by Real Madrid with 87 and Atletico with 85 with one match remaining.
Atletico took the lead at Levante in the first minute through Fernando Torres, but the relegated home side fought back, equalising through Victor Casadesus before Giuseppe Rossi grabbed the winner in stoppage time.