Harry Kane sustained ankle ligament damage in Sunday's 6-0 FA Cup win over Millwall.
Kane suffered the injury to his right ankle, and is the same knock as he picked up in September that ruled him out of action for seven weeks and 10 games.
Spurs are optimistic Kane's injury is not as severe this time.
"We can confirm that Harry Kane sustained ligament damage to his right ankle during Sunday's FA Cup win against Millwall," Spurs said on their Twitter account. "The injury is similar to the one suffered by the forward against Sunderland in September last year, however it is not considered to be as severe.
"Harry will continue to be assessed by our medical staff while he undergoes his rehabilitation at our Training Centre."