Lionel Messi suffered a groin injury and will be out at least three weeks.
“To lose Messi means that football loses. With Messi we are stronger, but we will continue being strong,” said Luis Enrique.
Barcelona tied Atletico Madrid 1-1 in a critical early season La Liga match.
Messi also missed several weeks early in the 15-16 season but Barcelona still went on to win La Liga.